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KMID : 0380619920240030204
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Korean Journal of Food Science and Technology
1992 Volume.24 No. 3 p.204 ~ p.208
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Development of Immunoassay Systems for the Assay of Soy Protein in Meat Products
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Abstract
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This study was carried out to develop a practical enzyme-linked immunosorbent assay(ELISA) for the determination of soy protein in processed meat products as a preliminary study. The titer of antiserum raised in rabbit by injection of SDS-treated whole buffer extract(WBE) from isolates soy protein(ISP) was above 1:10,000 in indirect ELISA. When the SDS concentration was higher than 0.03% the antibody-antigen reaction was inhibited significantly. However, the antibody-antigen reaction inhibition was not observed when the SDS concentration was less than 0.02%. The antibodies used in this experiment also reacted with renatured antigen after removing SDS by dialysis, though not better than with SDS-denatured antigen(immunogen). The calibration curve with 100 μg/100 ml of sensitivity was obtained in indirect competitive ELISA.
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